《植物生理学报》 2011, 47(4): 373-378

‘津田’ 芜菁Transparent Testa Glabra 1 (BrTTG1)基因的克隆及表达分析

闫海芳, 李玉花*, 刘振华, 闵远琴

东北林业大学生命科学学院, 哈尔滨150040

通信作者：李玉花；E-mail: lyhshen@126. com;；Tel: 0451-82191783

摘　要：

TTG1 (Transparent Testa Glabra 1)蛋白是一种WD40类蛋白, 参与植物的生长和发育。采用RT-PCR方法从芜菁品种‘津田’中克隆了BrTTG1 cDNA序列(GenBank登录号HM208590)。该基因cDNA开放阅读框长度为1 014 bp, 编码一个由337 个氨基酸残基组成的蛋白, 该蛋白分子量为37.28 kDa, 理论等电点为4.66。与其他植物中的TTG1蛋白进行同源性比对结 果显示, BrTTG1与甘蓝型油菜的TTG1同源性最高。BrTTG1蛋白在31~337位氨基酸处含有WD40超家族的保守结构域。 荧光定量PCR检测BrTTG1在‘津田’芜菁不同组织中的表达结果表明, 该基因在有花青素合成的红色‘津田’芜菁根皮中表达量最高。

关键词：‘津田’芜菁; TTG1; 基因克隆; 表达分析

收稿：2010-12-14   修定：2011-01-26

资助：国家自然科学基金重点项目(30730078)和中央高校基本科 研业务费专项资金(DL09BA09)。

Cloning and Expression Analysis of Transparent Testa Glabra 1 (BrTTG1) in Brassica rapa L. cv. ‘Tsuda’

YAN Hai-Fang, LI Yu-Hua*, LIU Zhen-Hua, MIN Yuan-Qin

College of Life Sciences, Northeast Forestry University, Harbin 150040, China

Corresponding author: LI Yu-Hua; E-mail: lyhshen@126. com;; Tel: 0451-82191783

Abstract:

TTG1 (transparent testa glabra 1) is a WD40 protein that is involved in plant growth and development. Here, a homologue of transparent testa glabra 1 gene, designated BrTTG1, was isolated from ‘Tsuda’ (Brassica rapa L.) by RT-PCR (GenBank accession number HM208590). The ORF of the BrTTG1 was 1 014 bp long and encoded a putative protein of 337 amino acids with a molecular weight of 37.28 kDa and a theoretical pI of 4.66. BrTTG1 protein contained a WD40 superfamily conserved domain and had a high identity with TTG1 homologous member from Brassica napus. Quantitative-PCR analysis showed that the BrTTG1 was the highest expressed in the in red root of turnip.

Key words: Brassica rapa L. cv. ‘Tsuda’; TTG1; gene cloning; expressing analysis